Use of a novobiocin-containing medium for isolation of Staphylococcus saprophyticus from urine.
نویسندگان
چکیده
The use of a novobiocin-containing medium provided little benefit over observable quantitative growth on blood agar in detecting Staphylococcus saprophyticus in urine cultures.
منابع مشابه
Abbreviated scheme for presumptive identification of Staphylococcus saprophyticus from urine cultures.
A simplified scheme for the presumptive identification of Staphylococcus saprophyticus was investigated. Clinical isolates of Micrococcaceae were tested in microtiter plates containing phenol red-novobiocin-turanose broth and phenol red-arabinose broth. Standard saline suspensions of test organisms served as inoculum. Identifying features were growth within 18 to 24 h in novobiocin-turanose bro...
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Of 16,347 urine cultures submitted to the hospital laboratory, 68 (0.4%) specimens from 50 patients yielded greater than 10(4) coagulase-negative staphylococci/ml in pure culture. A total of 62 of 63 organisms available for study were staphylococci: 45 Staphylococcus epidermidis (predominantly subgroup 1), 15 Staphylococcus saprophyticus (subgroup 3), and 2 Staphylococcus aureus. Twenty-one pat...
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The occurrence of Staphylococcus saprophyticus, Chlamydia trachomatis, and Neisseria gonorrhoeae in urethral specimens of 252 men attending a venereal disease clinic was studied. When using a selective broth medium containing novobiocin and nalidixic acid, Staph. saprophyticus was isolated from 20.8% of 178 men with symptoms of urethritis and from 14.9% of 74 men without such symptoms. Staph. s...
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Phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. Phosphatase-negative staphylococci, such as Staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as Staphylococcus epidermidis, by spot testing colonies on filter paper saturated with 1 N NaOH. Phosphatase-positive colonies turned ...
متن کاملColonization of the female genital tract with Staphylococcus saprophyticus.
The prevalence of colonization by Staphylococcus saprophyticus of the urogenital tracts of 276 women from an outpatient gynecology practice was determined by using selective and enrichment culture techniques. Nineteen subjects (6.9%) were found to be colonized by S. saprophyticus. The rectum was the most frequent site of colonization and was responsible for 40% of the isolates; this was followe...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 17 6 شماره
صفحات -
تاریخ انتشار 1983